Two DNA Methyltransferases for Site-Specific 6mA and 5mC DNA Modification in Xanthomonas euvesicatoria
نویسندگان
چکیده
منابع مشابه
Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleases.
We present in Table I an updated list of the sensitivities of over 240 restriction endonucleases to the site-specific DNA modifications '14C, n-6C, h1"5C, and m6A, four modifications that are common in DNA prokaryotes, eukaryotes, and their viruses (Mc2,Mc5,Mc8,Mcl 1 ,Ne3,Ne4). Table II is a list of over 130 characterized DNA methyltransferases. A detailed list of cloned restrictionmodification...
متن کاملEffect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases
Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known site-specific DNA modification methyltr...
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The mom gene of bacteriophage Mu encodes an enzyme that converts adenine to N(6)-(1-acetamido)-adenine in the phage DNA and thereby protects the viral genome from cleavage by a wide variety of restriction endonucleases. Mu-like prophage sequences present in Haemophilus influenzae Rd (FluMu), Neisseria meningitidis type A strain Z2491 (Pnme1) and H. influenzae biotype aegyptius ATCC 11116 do not...
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In mammalian cells, DNA methylation on the fifth position of cytosine (5mC) plays an important role as an epigenetic mark. However, DNA methylation was considered to be absent in C. elegans because of the lack of detectable 5mC, as well as homologs of the cytosine DNA methyltransferases. Here, using multiple approaches, we demonstrate the presence of adenine N(6)-methylation (6mA) in C. elegans...
متن کاملChimeric DNA methyltransferases target DNA methylation to specific DNA sequences and repress expression of target genes
Gene silencing by targeted DNA methylation has potential applications in basic research and therapy. To establish targeted methylation in human cell lines, the catalytic domains (CDs) of mouse Dnmt3a and Dnmt3b DNA methyltransferases (MTases) were fused to different DNA binding domains (DBD) of GAL4 and an engineered Cys2His2 zinc finger domain. We demonstrated that (i) Dense DNA methylation ca...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2021
ISSN: 1664-462X
DOI: 10.3389/fpls.2021.621466